Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.067206 |
Chromosome: | chromosome 10 |
Location: | 4673491 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g453350 | (1 of 1) PF00097//PF04536 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4) // TLP18.3, Psb32 and MOLO-1 founding proteins of phosphatase (TPM) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGCTCACTGCACGACCGCTGGGGCGTG |
Internal bar code: | TGGTGAGAGATAAGGGGATATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 829 |
LEAP-Seq percent confirming: | 99.5023 |
LEAP-Seq n confirming: | 2799 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGACGGCTACATTGGACT |
Suggested primer 2: | CCCACACACTCATCTGCATC |