Insertion junction: LMJ.RY0402.067206_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.067206
Chromosome:chromosome 10
Location:4673491
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g453350 (1 of 1) PF00097//PF04536 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4) // TLP18.3, Psb32 and MOLO-1 founding proteins of phosphatase (TPM) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCGCTCACTGCACGACCGCTGGGGCGTG
Internal bar code:TGGTGAGAGATAAGGGGATATC

Confirmation - LEAP-Seq

LEAP-Seq distance:829
LEAP-Seq percent confirming:99.5023
LEAP-Seq n confirming:2799
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGACGGCTACATTGGACT
Suggested primer 2:CCCACACACTCATCTGCATC