| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.067228 |
| Chromosome: | chromosome 9 |
| Location: | 7735125 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416050 | AGS1 | Argininosuccinate synthase; (1 of 1) 6.3.4.5 - Argininosuccinate synthase / Citrulline--aspartate ligase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGCCAGGATTGGATAATGGGAGGACA |
| Internal bar code: | GAGAAGGCCGGTCCCCTCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 76 |
| LEAP-Seq percent confirming: | 66.8781 |
| LEAP-Seq n confirming: | 3057 |
| LEAP-Seq n nonconfirming: | 1514 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACGCAGTGACACGAGTT |
| Suggested primer 2: | AGCCGGTTACGTTGGTACAC |