Insertion junction: LMJ.RY0402.067262_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.067262
Chromosome:chromosome 17
Location:828
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCATGCATGCGCAATCTGTCAACACAGCA
Internal bar code:GAGAGGCTCTGGTGATCGCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:8
LEAP-Seq percent confirming:45.1625
LEAP-Seq n confirming:1209
LEAP-Seq n nonconfirming:1468
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGTGCCAACCATACAACG
Suggested primer 2:AAAACCCTAAAACCCTAAAACCC