| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067276 |
| Chromosome: | chromosome 12 |
| Location: | 1907968 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGATGGTAGAGCAGGACAAGCCCTTGCG |
| Internal bar code: | GCGTATTGCTCGTCAGGCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 65.3846 |
| LEAP-Seq n confirming: | 1343 |
| LEAP-Seq n nonconfirming: | 711 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAGTAAAGCCCAAGTAG |
| Suggested primer 2: | GCTGTGGGATGCTAGTAGGC |