Insertion junction: LMJ.RY0402.067320_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.067320
Chromosome:chromosome 17
Location:2135016
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g712600 (1 of 726) IPR011009 - Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGACGAACGTCGACAAGAACGCTCACC
Internal bar code:CGGCGAAGATTAATATTACGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:612
LEAP-Seq percent confirming:99.9027
LEAP-Seq n confirming:2053
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGCCCCAACTACGTCAT
Suggested primer 2:ATTGCGATGGTCATTTGTGA