| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067332 |
| Chromosome: | chromosome 3 |
| Location: | 2275830 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158000 | GSAT,GSA,GSA1 | Glutamate-1-semialdehyde aminotransferase; (1 of 1) 5.4.3.8 - Glutamate-1-semialdehyde 2,1-aminomutase / Glutamate-1-semialdehyde aminotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGCAGAGCGCTCAATACTGGGCCTAT |
| Internal bar code: | ATCGCGGAGTCGGGGCTAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 99.5204 |
| LEAP-Seq n confirming: | 1245 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGAGCACCTGGAGAAGG |
| Suggested primer 2: | CAGTCATGCAGCCTACTCCA |