Insertion junction: LMJ.RY0402.067344_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.067344
Chromosome:chromosome 7
Location:2210386
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g327150 (1 of 2) IPR001810//IPR003347 - F-box domain // JmjC domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGCCGTCGCTGTCGCTGTCGCTGTCGTC
Internal bar code:TTCGGTCTGTTAAGCGCCCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:523
LEAP-Seq percent confirming:51.6836
LEAP-Seq n confirming:3822
LEAP-Seq n nonconfirming:3573
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGTAGCACCACGTTTGAA
Suggested primer 2:TCTGGGACAACACTCAACCA