| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.067345 |
| Chromosome: | chromosome 4 |
| Location: | 2630567 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222950 | SUR2 | (1 of 1) PTHR34348:SF1 - SURFEIT LOCUS PROTEIN 2; Surfeit 2-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTGGCTGCCGCCTGGCGAGGGGGTAGC |
| Internal bar code: | GGAGGTGTGCGAACCACTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 99.501 |
| LEAP-Seq n confirming: | 1994 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGACTGTTGAGGGGCTC |
| Suggested primer 2: | GCCGAAGTACCTGTGTAGCC |