| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.067350 |
| Chromosome: | chromosome 16 |
| Location: | 2063325 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657300 | CPI1 | Cyclopropyl isomerase-like protein; (1 of 1) 5.5.1.9 - Cycloeucalenol cycloisomerase / Cycloeucalenol--obtusifoliol isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTTGTCCAAGGGCCCATGCTGGGCAATG |
| Internal bar code: | TTCATATCGGTACTCGGCTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 926 |
| LEAP-Seq percent confirming: | 99.7944 |
| LEAP-Seq n confirming: | 4369 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGGCATACAGGAAAGTG |
| Suggested primer 2: | TGTAGATGAAGAGCATGGCG |