Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.067357 |
Chromosome: | chromosome_10 |
Location: | 2320300 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g434800 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCGTGTGGTGCAAAACGGGTTGGGGCACTT |
Internal bar code: | CGGCTAGATTGGGAGCGGACTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 88 |
LEAP-Seq percent confirming: | 97.2222 |
LEAP-Seq n confirming: | 35 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCCTAACCCGCACAGTGTC |
Suggested primer 2: | TACCTTTCCCATCTTGCCTG |