Insertion junction: LMJ.RY0402.067372_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.067372
Chromosome:chromosome 6
Location:1049684
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g256400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATTGCGGACGGACTAAACACGCTTTCCT
Internal bar code:TGTTTCGCGTGTCTGGGGTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:235
LEAP-Seq percent confirming:72.0072
LEAP-Seq n confirming:1600
LEAP-Seq n nonconfirming:622
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCCACAGATTACGGACTA
Suggested primer 2:CAGGTAGCACAGGAACAGCA