| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067382 |
| Chromosome: | chromosome 3 |
| Location: | 8939909 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g208721 | CGL92 | (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor; predicted protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCGGCGGTCGCGGTGCTGCTGGCACT |
| Internal bar code: | GGGGCGCGCAGGGCAAGAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 255 |
| LEAP-Seq percent confirming: | 73.8095 |
| LEAP-Seq n confirming: | 496 |
| LEAP-Seq n nonconfirming: | 176 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGACCGCACGCAATAAT |
| Suggested primer 2: | CGTCGGCACTTCACTTCATA |