| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067389 |
| Chromosome: | chromosome 9 |
| Location: | 3161397 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387134 | (1 of 3) PF04379 - Protein of unknown function (DUF525) (DUF525) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATCGAAGAGCAGCCCGAGCCCCACCCC |
| Internal bar code: | TGAATTTGGGGACAGCGGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 99.8824 |
| LEAP-Seq n confirming: | 849 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCGTGAGAGTGACTGCCT |
| Suggested primer 2: | AGGTACCGTATCCAAGCACG |