| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067440 |
| Chromosome: | chromosome_1 |
| Location: | 4397745 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029750 | FAK2,FAK1,DAAK1 | ODA5-associated flagellar adenylate kinase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCAATCTCCTGCAAGCGAGGCAATAGAAG |
| Internal bar code: | TGGACGGGAGAAGCGTTAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 345 |
| LEAP-Seq percent confirming: | 99.3464 |
| LEAP-Seq n confirming: | 760 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGAACAAACGTGCAATC |
| Suggested primer 2: | GGCGTCCGTTCATACTGTTT |