| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.067441 |
| Chromosome: | chromosome 9 |
| Location: | 2862720 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTGTCGGCGCTGGATGCGCTCGCTCGG |
| Internal bar code: | CGGTGACGTTAGGGTTGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 47.06 |
| LEAP-Seq n confirming: | 2409 |
| LEAP-Seq n nonconfirming: | 2710 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACTGGGTTTGGATCGGA |
| Suggested primer 2: | AGGGTGCACTGGTAGGATTG |