| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.067467 |
| Chromosome: | chromosome 11 |
| Location: | 1166164 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467688 | (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCAGGGTTTATATTTAGCCTCAAGCAG |
| Internal bar code: | AGGCTCACAGAGGCGTGGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 99.1868 |
| LEAP-Seq n confirming: | 4879 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGTACCCTCCGAAGAT |
| Suggested primer 2: | AGGCTAGCGAGTGGAAAACA |