| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.067469 |
| Chromosome: | chromosome 3 |
| Location: | 3706518 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169450 | (1 of 7) IPR000048//IPR027417 - IQ motif, EF-hand binding site // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAATACCTGCCTACGTACACGCTCTGC |
| Internal bar code: | AAAGCCCGTAGGTCGTGTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1222 |
| LEAP-Seq percent confirming: | 99.6859 |
| LEAP-Seq n confirming: | 2539 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGGTATGGTTTCTGGAC |
| Suggested primer 2: | TGATGAGCTCACGGAGTGAC |