| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.067470 |
| Chromosome: | chromosome 12 |
| Location: | 7269023 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557900 | HYD1,CDI1 | C-8%252C7 sterol isomerase; (1 of 1) 5.3.3.5 - Cholestenol Delta-isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGGCTGAAATGAATCGTTGAATCCACC |
| Internal bar code: | ATCGGAGGCTGGGCGACTAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 99.784 |
| LEAP-Seq n confirming: | 924 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACGGACTCTTTCCAACGG |
| Suggested primer 2: | GTCAGGGTCAAGCTCCTCTG |