| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.067471 |
| Chromosome: | chromosome 16 |
| Location: | 7245617 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682475 | (1 of 1) PTHR34496//PTHR34496:SF1 - FAMILY NOT NAMED // GLCNAC TRANSFERASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGCGAAGTTGTAGCCAGGACCAGGGGG |
| Internal bar code: | CTTTGTTTCGAACTGTGTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 97.1622 |
| LEAP-Seq n confirming: | 1575 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTAGCACAAATCAGCAA |
| Suggested primer 2: | ATTTGAGCTTGAAGGCAGGA |