| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067476 |
| Chromosome: | chromosome 12 |
| Location: | 2830525 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502450 | ELG8 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 8 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTCCCTTTAGGCTTGATCTGAGTCGGC |
| Internal bar code: | ATCGAGACTCGGGGGGTGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 68.9855 |
| LEAP-Seq n confirming: | 238 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTATGACATCGTGTTTGG |
| Suggested primer 2: | ACAGTGCTCTGCCCGTAAGT |