Insertion junction: LMJ.RY0402.067478_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.067478
Chromosome:chromosome_14
Location:864277
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre14.g613400 POC16 Centriole proteome protein antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GGGGGTGGGCAGTGGCGGCGTTGGGTTGGT
Internal bar code:GAGAAGATCTAAGCGCGCCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:847
LEAP-Seq percent confirming:98.1887
LEAP-Seq n confirming:1301
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCCAAACCCAACAATCTG
Suggested primer 2:TGCTTACATCATCCTCGCAG