| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.067509 |
| Chromosome: | chromosome 12 |
| Location: | 2407772 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507200 | (1 of 2) IPR000104//IPR003613 - Antifreeze protein, type I // U box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGCGGATAAATGGGCTTGAGTGGCATG |
| Internal bar code: | GAGTCGCGACTCCGGTCAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 94.393 |
| LEAP-Seq n confirming: | 5606 |
| LEAP-Seq n nonconfirming: | 333 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGGCGTTGCGTCTATAA |
| Suggested primer 2: | TGTGTTCATTGCTCGAAAGC |