| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.067512 |
| Chromosome: | chromosome 12 |
| Location: | 9485377 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540076 | CDC68 | (1 of 1) PTHR13980:SF15 - FACT COMPLEX SUBUNIT SPT16 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACAGCTGAGCCCCATGCGCCATTGCTTT |
| Internal bar code: | TGTCACTTGTCATTCACGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 81.3562 |
| LEAP-Seq n confirming: | 5315 |
| LEAP-Seq n nonconfirming: | 1218 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCTCGTCCCAGTCCTTG |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |