| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.067515 |
| Chromosome: | chromosome 4 |
| Location: | 98791 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216600 | RPT6 | (1 of 1) K03066 - 26S proteasome regulatory subunit T6 (PSMC5, RPT6); 26S proteasome regulatory subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAACCCCCGCACTTCACTTTGTCATGGA |
| Internal bar code: | TTGTTAACGCCCGCCGAACTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3 |
| LEAP-Seq percent confirming: | 99.7543 |
| LEAP-Seq n confirming: | 812 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGTAAGACGAAGGTGGA |
| Suggested primer 2: | GTACTTCTGGACCAGCTCGC |