Insertion junction: LMJ.RY0402.067529_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.067529
Chromosome:chromosome 9
Location:4982644
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g397993 FAP201 (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype; Leucine Rich Repeat Flagellar Associated Protein 201 gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAACAGAACTAAGCGAAGATTAGCATGGC
Internal bar code:GTGGCAGTGCTTCCGGCCTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:380
LEAP-Seq percent confirming:97.931
LEAP-Seq n confirming:142
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTGAGAGCTAGGAGGCA
Suggested primer 2:GACTAGGCAGAAGCACCAGG