| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.067536 |
| Chromosome: | chromosome 13 |
| Location: | 1677122 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574050 | (1 of 6) PTHR10994//PTHR10994:SF72 - RETICULON // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCTAAGGCTCATGAGGAAGACCGGCAC |
| Internal bar code: | CTCAGGCCCGCTGCTAATCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.8172 |
| LEAP-Seq n confirming: | 4915 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGCTCCGGTTGTTAAT |
| Suggested primer 2: | ATGTTGAGTGGAGGCAGCTT |