| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067561 |
| Chromosome: | chromosome 2 |
| Location: | 4907497 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103500 | (1 of 1) 1.14.13.169 - Transferred entry: 1.14.18.5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGCATTTAGGCACACAGGTTATGGGAG |
| Internal bar code: | GCGGATTGGGGCATAAAGCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 901 |
| LEAP-Seq percent confirming: | 99.0039 |
| LEAP-Seq n confirming: | 12821 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCATGGACACGTGGCAGTA |
| Suggested primer 2: | TCGCTCATGCTTGTAACTGG |