Insertion junction: LMJ.RY0402.067581_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.067581
Chromosome:chromosome 8
Location:856071
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g361150 GT90F11,GT90-11 GT90 family protein 11; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGTAGTACTCGATCCAGGGGGAGCGCTC
Internal bar code:GAGACGCCGTTCAAAGCAAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:293
LEAP-Seq percent confirming:83.4171
LEAP-Seq n confirming:498
LEAP-Seq n nonconfirming:99
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACACACACGGT
Suggested primer 2:CGCTCCACTTCAACCTCTTC