| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.067636 |
| Chromosome: | chromosome 9 |
| Location: | 7747213 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416200 | HCF107,MBB1 | PsbB mRNA maturation factor; (1 of 1) PTHR11246//PTHR11246:SF11 - PRE-MRNA SPLICING FACTOR // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCCGTACGCCTGCCACATGTACAGGTC |
| Internal bar code: | CCGCTGCCATCCGGCGAAGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1230 |
| LEAP-Seq percent confirming: | 99.285 |
| LEAP-Seq n confirming: | 1944 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAATCGTTACCTACGCC |
| Suggested primer 2: | ATGGAGTGCTATCACCAGGG |