| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.067640 |
| Chromosome: | chromosome 7 |
| Location: | 5522415 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351100 | THB5,PRX | (1 of 1) IPR000104//IPR001486//IPR009050 - Antifreeze protein, type I // Truncated hemoglobin // Globin-like; Truncated hemoglobin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTTGCGGAGCCAACGCCACCAGGCGCT |
| Internal bar code: | ATCGCGTCAAGATTGAGGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 87.5349 |
| LEAP-Seq n confirming: | 2507 |
| LEAP-Seq n nonconfirming: | 357 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATCCAAACCAAAATGAC |
| Suggested primer 2: | CTCCAGGATTCGCTTGTGAT |