| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067660 |
| Chromosome: | chromosome 5 |
| Location: | 3021349 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238500 | (1 of 4) K06941 - 23S rRNA (adenine2503-C2)-methyltransferase [EC:2.1.1.192] (rlmN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCACCTCCTCCTCCGCCGCCGCCAGCT |
| Internal bar code: | GGGTTAGACGGGACGTCCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 97.1066 |
| LEAP-Seq n confirming: | 61082 |
| LEAP-Seq n nonconfirming: | 1820 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACATAGGCTCTGGCTCTG |
| Suggested primer 2: | TCCTCGTTAACACCCACACA |