| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067719 |
| Chromosome: | chromosome 14 |
| Location: | 3282190 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629650 | NIK1 | Nickel transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGAGGTGAGGTGGGTCGAGGGTTGGA |
| Internal bar code: | GGCCACCACCGAGTACCTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 60.6742 |
| LEAP-Seq n confirming: | 540 |
| LEAP-Seq n nonconfirming: | 350 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGACAGTCGTGTTCAGTC |
| Suggested primer 2: | TTAACTCGGCCCTTAACACG |