Insertion junction: LMJ.RY0402.067731_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.067731
Chromosome:chromosome 10
Location:4604733
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g452750 TPT18,TPT16 UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 3) PTHR11132//PTHR11132:SF125 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGCCCACCAGCCCACCCGCCCTCTATCC
Internal bar code:CTTCATGGCTGACTCATCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:757
LEAP-Seq percent confirming:99.7785
LEAP-Seq n confirming:901
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGCTGTAGATCTGGGAAT
Suggested primer 2:ATCACAATCAAGCGACCCTC