| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.067734 |
| Chromosome: | chromosome 17 |
| Location: | 2696549 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g717919 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTTTGGCCGGGTGCGTGTGCGGGCTGC |
| Internal bar code: | ATGCCGGTCTAGGGGGGCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 98.8165 |
| LEAP-Seq n confirming: | 8266 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTGCCTCTCACAACAAG |
| Suggested primer 2: | TTCGACAGCACCAAGAACTG |