| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067742 |
| Chromosome: | chromosome 3 |
| Location: | 8216179 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199800 | HYD1,HYDA1 | (1 of 2) 1.12.7.2 - Ferredoxin hydrogenase / Uptake hydrogenase; Iron hydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTAGTAGCTATGCCGTTCTTTTACAGGC |
| Internal bar code: | AGGCCAAGCTGGCGACGGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 89 |
| LEAP-Seq percent confirming: | 79.7203 |
| LEAP-Seq n confirming: | 114 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGAGGGATTGAGGAGAAT |
| Suggested primer 2: | ACTGGCCTTGGATAATGCAC |