| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.067753 |
| Chromosome: | chromosome 9 |
| Location: | 7939964 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g417350 | Macrocin O-methyltransferase; (1 of 1) 2.1.1.285 - Demethyldecarbamoylnovobiocin O-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCAGCACGCACACATGCAGCCCCAAACA |
| Internal bar code: | GTATCTACATGCCATCATGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1075 |
| LEAP-Seq percent confirming: | 99.3596 |
| LEAP-Seq n confirming: | 4810 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAATGTTGTTGTGGCTG |
| Suggested primer 2: | AAAGTAAGTTTCCCGGCGTT |