Insertion junction: LMJ.RY0402.067758_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.067758
Chromosome:chromosome 3
Location:4060838
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g172550 PRM1,PRMT1 (1 of 2) K11434 - protein arginine N-methyltransferase 1 (PRMT1); Protein-/Histone-arginine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACACACGAGCCCGCGGAACGGGGAGTCGG
Internal bar code:TGTCCCATGCCCCCCGCTCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:55
LEAP-Seq percent confirming:84.3552
LEAP-Seq n confirming:1197
LEAP-Seq n nonconfirming:222
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACATCGAAGAAACCCACCTG
Suggested primer 2:TACGGCTTCAACATGAGCTG