Insertion junction: LMJ.RY0402.067778_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.067778
Chromosome:chromosome 2
Location:1687207
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g085850 ARS6 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGGGGGCGAGGGGCGGGACGGGGTGCCG
Internal bar code:ACCCCCCGTCGAATTCCGTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:345
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTACACCTCCGACAACGGAT
Suggested primer 2:TGCGTTTGTGCTCCTAAATG