Insertion junction: LMJ.RY0402.067778_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.067778
Chromosome:chromosome 2
Location:1687207
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g085850 ARS6 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGGGGGCGAGGGGCGGGACGGGGTGCCG
Internal bar code:ACACCCCGTCGAATTCCGTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:369
LEAP-Seq percent confirming:93.2232
LEAP-Seq n confirming:11968
LEAP-Seq n nonconfirming:870
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CTACACCTCCGACAACGGAT
Suggested primer 2:TGCGTTTGTGCTCCTAAATG