| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067788 |
| Chromosome: | chromosome_14 |
| Location: | 3612380 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g631200 | DII6,IC97,FAP94 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGAGAAGGTCTTGGAGGCGCCGTCGAACGC |
| Internal bar code: | AAACGGGGGAATTGCAACGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 124 |
| LEAP-Seq percent confirming: | 98.7952 |
| LEAP-Seq n confirming: | 164 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTTAGTTTGGGCTGGTA |
| Suggested primer 2: | CGCCCTAACTCTTGTTCCTG |