| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067833 |
| Chromosome: | chromosome 15 |
| Location: | 206211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635300 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTGAAGTTCGGAGAAGCACTACTTACT |
| Internal bar code: | AGCAGGCTCTCACTCGATGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 99.6377 |
| LEAP-Seq n confirming: | 275 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGCTAGCTTGAGGTTTG |
| Suggested primer 2: | ATCGGACGAAACGTGATAGG |