Insertion junction: LMJ.RY0402.067846_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.067846
Chromosome:chromosome 13
Location:3741449
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 5'UTR/CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGACGATTGAGGTTGGCGACTGGCTCTCC
Internal bar code:TTCTCCCTCGTCCGGATCCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:712
LEAP-Seq percent confirming:99.2992
LEAP-Seq n confirming:3259
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AACATGCCTGGTTGTAAGCC
Suggested primer 2:GAGGTAGCTGAACTCCACCG