| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067850 |
| Chromosome: | chromosome_2 |
| Location: | 2547527 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g092700 | FAP45 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAAACTTCGATGGGTTCAGGACCCCCGCTG |
| Internal bar code: | CATGGTGCTCCCGTTTGCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 872 |
| LEAP-Seq percent confirming: | 97.7358 |
| LEAP-Seq n confirming: | 1036 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATAATGCGGCGTTCTGT |
| Suggested primer 2: | ACAAACCTTGTGATGTGGCA |