| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.067854 |
| Chromosome: | scaffold 40 |
| Location: | 10808 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre40.g760197 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCGGAGCCGGGCCCACAACGGGGGGTC |
| Internal bar code: | GTGCCGGGATTTTGTTCTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 98.1395 |
| LEAP-Seq n confirming: | 633 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTGTTGAACCACTCAA |
| Suggested primer 2: | GCCAGCCACTCTCAACCTAC |