Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.067920 |
Chromosome: | chromosome_5 |
Location: | 2298960 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g234700 | PYK3 | Pyruvate kinase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTTAGGGAGCGGTGAGTCCAGCACCCTGC |
Internal bar code: | GGGTTGGCGTTGTTTATTGGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 964 |
LEAP-Seq percent confirming: | 98.9696 |
LEAP-Seq n confirming: | 2017 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGACCAATTGATGTGGTTG |
Suggested primer 2: | GTCGATGATCTCGTCCAGGT |