Insertion junction: LMJ.RY0402.067922_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.067922
Chromosome:chromosome 2
Location:2821066
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g094551 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGCCCACTGCCGCTCCCGTCGTCGGGA
Internal bar code:GCGCCCGCGAGTGAGGTTAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:65
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCCATCTTCAGTAAGCTC
Suggested primer 2:CCAACACGGAACGATACCTT