| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.067980 |
| Chromosome: | chromosome 12 |
| Location: | 717942 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495050 | OPR51 | OctotricoPeptide Repeat protein 51 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGTAGGGAACTAGGGGTCCATGCAGC |
| Internal bar code: | GGACCGTGAGTGAACGGCAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 91.6196 |
| LEAP-Seq n confirming: | 973 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGCCACTCGTACTGTGC |
| Suggested primer 2: | AGTCTTTGTTGCTGCTGCTG |