| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068009 |
| Chromosome: | chromosome_12 |
| Location: | 8687035 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g546400 | DLR2,DLC7b,DLC7B | Flagellar inner arm I1/f axonemal dynein, LC7b | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGGATATGAGAACGTTTAGAGCTGGGCCG |
| Internal bar code: | TGCGGTTACGGGGTTAAGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 569 |
| LEAP-Seq percent confirming: | 88.3756 |
| LEAP-Seq n confirming: | 2372 |
| LEAP-Seq n nonconfirming: | 312 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCCAAGCGGTGACAGAC |
| Suggested primer 2: | GTACTATGGATGCAGGGCGT |