| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.068042 |
| Chromosome: | chromosome 3 |
| Location: | 5816219 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189100 | (1 of 1) PTHR31606:SF1 - WBP2 ORTHOLOG, ISOFORM E | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAAGCATGCAAGCAGGCGGTGGAATGAT |
| Internal bar code: | GAGCTAGCCGTTTGCTAATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 918 |
| LEAP-Seq percent confirming: | 99.009 |
| LEAP-Seq n confirming: | 5295 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATTAGTGTCCACACGCT |
| Suggested primer 2: | ACTTGTTCCAGAATGACGGC |