| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068061 |
| Chromosome: | chromosome 3 |
| Location: | 7569553 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204800 | RTA2 | (1 of 2) 3.6.3.16 - Arsenite-transporting ATPase / Arsenite-translocating ATPase; Arsenite-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGAGGCTATGTGGAGCGTTGGGGTATC |
| Internal bar code: | CAGTACGCCCGTCATTCAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 47.321 |
| LEAP-Seq n confirming: | 1104 |
| LEAP-Seq n nonconfirming: | 1229 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAGTTCCTGTCGCTCTAC |
| Suggested primer 2: | CCTTGCCTACAGCTAGCACC |